Protozoan parasites cause a wide range of devastating diseases in various kinds of organisms, including humans. It may be lethal if untreated promptly. To detect specific disease-causingorganisms parasites, a wide range of immunological and molecular technologies are now widely available. However, all of this depends on the worker's expertise and are time-consuming, error-prone, and expensive. With the development of technology, compared to traditional biological techniques, convolutional neural networks have reached excellent achievements in image classification, cutting costs while attaining an overall higher accuracy and eliminating human error. Many models include numerous convolutional layers and offer an accuracy between 90 and 95 percent. In this study, 4740 microscopic images of protozoan parasites from six classes with a balanced dataset and an 80–20% split were classified using three convolutional layers with stochastic gradient descent as an optimizer. A 5-fold cross-validation approach is used to evaluate the proposed method. We also examine and evaluate with deep learning models namely VGG16, ResNet50, and InceptionV3. The performance evaluation of the proposed model shows an accuracy of 94% with a precision range (of 0.83-0.99) and a recall range (of 0.76-1.00), respectively. The retrained model was able to recognize and classify all 6 different parasites. Except for class Leishmania, where 24% of images are incorrectly classified as Plasmodium and Trichomonas, the model demonstrates that most cases are correctly identified.

Protozoan parasites, Convolutional neural networks, Multi-class classification, Microscopic images, Image analysis